Season: 2025
| # | Team | MP | W | D | L | GF | GA | GD | Pts |
|---|---|---|---|---|---|---|---|---|---|
| 1 |  FC Cincinnati II | 28 | 18 | 0 | 10 | 47 | 34 | 13 | 54 |
| 2 |  Bethlehem Steel | 28 | 18 | 0 | 10 | 59 | 41 | 18 | 52 |
| 3 |  Chicago Fire | 28 | 16 | 0 | 12 | 51 | 51 | 0 | 47 |
| 4 |  New York City II | 28 | 14 | 0 | 14 | 58 | 46 | 12 | 45 |
| 5 |  Columbus Crew II | 28 | 15 | 0 | 13 | 53 | 47 | 6 | 45 |
| 6 |  New York RB II | 28 | 14 | 0 | 14 | 56 | 61 | -5 | 40 |
| 7 |  Toronto II | 28 | 11 | 0 | 17 | 44 | 51 | -7 | 37 |
| 8 |  New England II | 28 | 8 | 0 | 20 | 37 | 59 | -22 | 22 |
| 1 |  Fort Lauderdale CF | 28 | 14 | 0 | 14 | 53 | 45 | 8 | 48 |
| 2 |  Orlando City II | 28 | 15 | 0 | 13 | 53 | 42 | 11 | 46 |
| 3 |  Crown Legacy | 28 | 16 | 0 | 12 | 51 | 46 | 5 | 45 |
| 4 |  Chattanooga | 28 | 17 | 0 | 11 | 45 | 42 | 3 | 45 |
| 5 |  Carolina Core | 28 | 15 | 0 | 13 | 39 | 45 | -6 | 43 |
| 6 |  Atlanta United II | 28 | 11 | 0 | 17 | 42 | 64 | -22 | 32 |
| 7 |  Huntsville City | 28 | 8 | 0 | 20 | 39 | 53 | -14 | 29 |
| 1 |  San Jose Earthquakes II | 28 | 17 | 0 | 11 | 41 | 28 | 13 | 51 |
| 2 |  Tacoma Defiance | 28 | 15 | 0 | 13 | 59 | 53 | 6 | 46 |
| 3 |  Los Angeles II | 28 | 15 | 0 | 13 | 51 | 54 | -3 | 45 |
| 4 |  LA Galaxy II | 28 | 16 | 0 | 12 | 49 | 49 | 0 | 43 |
| 5 |  Whitecaps II | 28 | 13 | 0 | 15 | 45 | 44 | 1 | 40 |
| 6 |  Real Monarchs | 28 | 14 | 0 | 14 | 39 | 41 | -2 | 39 |
| 7 |  Portland Timbers II | 28 | 12 | 0 | 16 | 43 | 45 | -2 | 38 |
| 1 |  North Texas | 28 | 22 | 0 | 6 | 56 | 32 | 24 | 62 |
| 2 |  St. Louis City II | 28 | 18 | 0 | 10 | 53 | 35 | 18 | 56 |
| 3 |  Houston Dynamo FC II | 28 | 12 | 0 | 16 | 46 | 45 | 1 | 39 |
| 4 |  Swope Park Rangers | 28 | 12 | 0 | 16 | 53 | 57 | -4 | 38 |
| 5 |  Austin II | 28 | 11 | 0 | 17 | 44 | 49 | -5 | 36 |
| 6 |  Minnesota United II | 28 | 12 | 0 | 16 | 43 | 73 | -30 | 32 |
| 7 |  Colorado Rapids II | 28 | 7 | 0 | 21 | 37 | 54 | -17 | 23 |
